Complement Component Assays.
Biological Data Analysis. Bioinformatics Data Management. Custom Bioinformatics Software Development. Process Development Service. Biosimilar Testing Services. Recombinant Protein Expression in E. Recombinant Protein Expression in Insect Cells. Recombinant Protein Expression in Pichia Pastoris. Co-Immunoprecipitation Co-IP. Yeast Two-Hybrid Screening. Competition reactions contained fold molar excess of unlabeled oligo duplex.
Electrophoretic Mobility Shift Assay (EMSA) | Protocol
Arrow indicates mobility shift of fluorescent oligos. Asterisk indicates reduction of mobility shift caused by an excess of unlabeled competitor DNA.
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- Gel Shift Assays (EMSA) | Thermo Fisher Scientific - FI.
- Additional information.
Wet gel was imaged with Odyssey Classic Infrared Imager. Blocking, streptavidin incubation, and washes are required Gel can be replaced and run longer, if needed Gel run time cannot be extended Gel run time cannot be extended Results available in less than 2 hours Results typically not obtained until the next day Detection steps add several hours to protocol. Chat With Us. Got it Learn More. Indirect detection method.
Abstract We developed a new approach to prepare DNA probes for electrophoretic mobility gel shift assays that presents a number of advantages compared with the classical approach. Keywords: electrophoretic mobility shift assay EMSA gel shift. Competing interests The authors declare no competing interests.
References 1. Lane, D. Prentki, and M. Use of gel retardation to analyze protein-nucleic acid interactions. Laniel, M.
LUEGO: a cost and time saving gel shift procedure
Electrophoretic mobility shift assays for the analysis of DNA-protein interactions. Methods Mol. Hellman, L. Electrophoretic mobility shift assay EMSA for detecting protein-nucleic acid interactions.
Fluorescence-based Electrophoretic Mobility Shift Assay in the Analysis of DNA-binding Proteins
Protocols — Heidebrecht, F. Heidebrecht, I. Schulz, S. Behrens, and A. Improved semiquantitative Western blot technique with increased quantification range. Methods — Sagner, C. Kessler, and G. Sensitive chemiluminescent detection of digoxigenin-labled nucleic acids: a fast and simple protocol and its applications.
Biotechniques — Ruscher, K. Reuter, D. Kupper, G.
Trendelenburg, U. Dinargl, and A. A fluorescence based non-radioactive electrophoretic mobility shift assay. Ying, B. Fourmy, and S. Substitution of the use of radioactivity by fluorescence for biochemical studies of RNA. RNA — Anderson, B. Larkin, K.
Related electrophoretic mobility shift assay advantages
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